HPVomics

A therapeutic and epitome resource for Human Papillomaviruses

Efficient putative sgRNAs (CRISPR/Cas9 targets)

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S.No.	HPV type	Target region	sgRNA seuences	PAM	Strand(F/R)	Start	End	GC (%)	Predicted effeciency (%)
1	HPV16	E1^E4	GGCTTTGGTATGGGTCGCGG	CGG	R	188	207	65	54.64
2	HPV16	E1^E4	GCATTTAACAGCTCACACAA	AGG	F	222	241	40	48.36
3	HPV18	E1^E4	GGTGCTGGAATACGGTGAGG	GGG	R	176	195	60	59.3
4	HPV18	E1^E4	GGTGTAGCTGCACCGAGAAG	TGG	R	54	73	60	44.63
5	HPV18	E1^E4	GTATTCCAGCACCGTGTCCG	TGG	F	80	99	60	43.47
6	HPV18	E1^E4	CTACAGGCAACAACAAAAGA	CGG	F	214	233	40	41.86
7	HPV26	E1^E4	CGTGGGCACCAAGGAAACCG	AGG	F	101	120	65	55.03
8	HPV26	E1^E4	GGAAAGCGACGACGACTCAG	TGG	F	135	154	60	52.81
9	HPV26	E1^E4	GTAGGCTTCGGTGGTCTGGG	TGG	R	215	234	65	49.29
10	HPV26	E1^E4	CTACGTGCCCGTGGGCACCA	AGG	F	92	111	70	47.35
11	HPV26	E1^E4	GGCTTCGGTGGTCTGGGTGG	TGG	R	218	237	70	45.99
12	HPV31	E1^E4	GTTGTTGCGAGGCGACTCCG	TGG	F	194	213	65	54.6
13	HPV31	E1^E4	GGTTTTGGAATTCGATGTGG	TGG	R	206	225	45	50.99
14	HPV31	E1^E4	GCACCAGTGAAGGTGTGCGG	CGG	F	106	125	65	49.94
15	HPV33	E1^E4	CTAACTGCACAAACAAGCAG	CGG	F	190	209	45	53.82
16	HPV35	E1^E4	GCGAGTGCGACTCAGTGCCG	TGG	F	176	195	70	59.24
17	HPV35	E1^E4	CCATACCAAAGCCTGCTCCG	TGG	F	80	99	60	51.51
18	HPV35	E1^E4	GGCTTTGGTATGGGTCTCGG	TGG	R	191	210	60	50.19
19	HPV39	E1^E4	GGACACTCCGTCGGGCTCAG	TGG	R	89	108	70	51.73
20	HPV39	E1^E4	GTACAGGCCACAACACAAGA	CGG	F	223	242	50	47.44
21	HPV39	E1^E4	ACAGAGCCCACTGAGCCCGA	CGG	F	159	178	65	44.6
22	HPV39	E1^E4	GGATTGTTTTTTGGGTGCAT	GGG	R	157	176	40	42.89
23	HPV39	E1^E4	GGTGCATGGGGTTGTTGCGG	TGG	R	170	189	65	41.48
24	HPV45	E1^E4	GGACTCACAGAGCAGCACCA	CGG	F	156	175	60	78.02
25	HPV45	E1^E4	GTACAAGTAACAACAAAAGA	AGG	F	220	239	30	52.34
26	HPV45	E1^E4	CGTGGGTGTTGACACGTCCG	TGG	R	79	98	65	47.3
27	HPV45	E1^E4	TGTTACTTGTACTTGAACAC	AGG	R	43	62	35	42.16
28	HPV51	E1^E4	GGAAGTGGAAGCACTAACAC	TGG	F	210	229	50	58.58
29	HPV51	E1^E4	GGACAGTGGGGAGATTGTGG	AGG	R	89	108	60	45.6
30	HPV51	E1^E4	ACAAACAACCAAATACACTG	TGG	F	189	208	35	44.58
31	HPV51	E1^E4	CACAATCTCCCCACTGTCCG	TGG	F	161	180	60	41.12
32	HPV51	E1^E4	TTTGTATTGTCCACGGACAG	TGG	R	75	94	45	40.48
33	HPV52	E1^E4	GGACACTGCGGAGGTCTTGG	AGG	R	200	219	65	50.83
34	HPV52	E1^E4	GGCACCCACGGACACTGCGG	AGG	R	191	210	75	46.81
35	HPV52	E1^E4	TTTGCGGGGACAACAATCCG	TGG	F	179	198	55	46.54
36	HPV52	E1^E4	GGTCGTCGTCGTTTCTGTGG	TGG	R	150	169	60	44.22
37	HPV53	E1^E4	GGACTCTCTGGCTGTGGTAG	TGG	R	122	141	60	60.09
38	HPV53	E1^E4	GGGACGTTTTCCCGGCCGCA	GGG	R	185	204	70	58.07
39	HPV53	E1^E4	GGAGGTGGTGGGGGTCTTAT	GGG	R	239	258	60	54.72
40	HPV53	E1^E4	GGCTGTGGTAGTGGACTGTG	TGG	R	131	150	60	52.52
41	HPV53	E1^E4	AACAACACCGAAATCGACAG	TGG	F	285	304	45	48.73
42	HPV53	E1^E4	GGTGCCCACGGGCGTGGAGG	TGG	R	224	243	80	44.75
43	HPV53	E1^E4	TGTGGTAGTGGACTGTGTGG	TGG	R	134	153	55	43.15
44	HPV53	E1^E4	GTAGGTGCCCACGGGCGTGG	AGG	R	221	240	75	41.17
45	HPV56	E1^E4	GGACGTGGAGGTGGTGGTGG	TGG	R	200	219	70	56.67
46	HPV56	E1^E4	GGTGGTGGTGGTGGTCTTGT	GGG	R	209	228	60	42.36
47	HPV58	E1^E4	CCAACAACGAAAGTACACAG	GGG	F	67	86	45	59.61
48	HPV58	E1^E4	GCCGTGGACAGTAGACCACG	AGG	F	162	181	65	51.17
49	HPV58	E1^E4	TGCTGACCCAAAGACCACCG	AGG	F	41	60	60	50
50	HPV58	E1^E4	AAAGTATACAGACTGCGCCG	TGG	F	146	165	50	47.37
51	HPV58	E1^E4	CTAACTGTACATACAAAGGG	CGG	F	205	224	40	42.73
52	HPV59	E1^E4	GGAAAGCGACCAAGACAGTG	TGG	F	135	154	55	50.37
53	HPV59	E1^E4	ATAGGCGTAGTGTTACTGCA	AGG	R	3	22	45	47.03
54	HPV59	E1^E4	TTGCTCAGAAGATCCAGCAG	TGG	R	221	240	50	43.68
55	HPV66	E1^E4	TGTGGGCGCCCAAGACGCCG	CGG	F	98	117	75	51.41
56	HPV66	E1^E4	TCCTGGTCTGTGGGATACCG	CGG	R	155	174	60	47.42
57	HPV66	E1^E4	GGCGTCTTGGGCGCCCACAA	AGG	R	176	195	70	41.85
58	HPV66	E1^E4	TGGCTTTGTGTGTTGATACG	TGG	R	27	46	45	41.14
59	HPV68	E1^E4	GGACACGTTGTCGGGCTCAG	AGG	R	89	108	65	49.42
60	HPV68	E1^E4	GGTGCATGGGGCACCTGCGG	TGG	R	170	189	75	47.65
61	HPV68	E1^E4	TGAGCCCGACAACGTGTCCG	TGG	F	170	189	65	42.42
62	HPV68	E1^E4	TGGGAGGTCGACACCGTCCA	CGG	R	68	87	65	41.86
63	HPV73	E1^E4	GGTGTCAACGTTGGTGCAGG	TGG	R	128	147	60	53.45
64	HPV73	E1^E4	GGTGGTGGTGTTGGTGGTTG	TGG	R	149	168	60	50.36
65	HPV73	E1^E4	GGTGCAGGTGGTGGTGGTGT	TGG	R	140	159	65	42.12
66	HPV73	E1^E4	AGGTTATGCAAACAATCCAG	GGG	R	57	76	40	41.34
67	HPV82	E1^E4	GCAGGTGTCCACGGACAGCG	GGG	R	77	96	70	66.8
68	HPV82	E1^E4	GGAATAGGTATTGGTCGTGG	GGG	R	182	201	50	57.11
69	HPV82	E1^E4	CTGCAGGCCCAAACACCGGA	GGG	F	217	236	65	46.18
70	HPV82	E1^E4	GGACAGCGGGGTGACTGTGG	AGG	R	89	108	70	46.14
71	HPV82	E1^E4	GGGGTGACTGTGGAGGAGTC	GGG	R	96	115	65	44.55
72	HPV82	E1^E4	GGCGGAATAGGTATTGGTCG	TGG	R	179	198	55	44.05
73	HPV82	E1^E4	GGCTTCTGTGGTGCCCGCGG	AGG	R	158	177	75	43.72
74	HPV82	E1^E4	GGAACTGCAGGCCCAAACAC	CGG	F	213	232	60	41.43